Direct-Current Electrical Field Stimulation of Patient-Derived Colorectal Cancer Cells.

ABSTRACT: Several cues for a directional migration of colorectal cancer cells were identified as being crucial in tumor progression. However, galvanotaxis, the directional migration in direct-current electrical fields, has not been investigated so far. Therefore, we asked whether direct-current electrical fields could be used to mobilize colorectal cancer cells along field vectors. For this purpose, five patient-derived low-passage cell lines were exposed to field strengths of 150-250 V/m in vitro, and migration along the field vectors was investigated. To further study the role of voltage-gated calcium channels on galvanotaxis and intracellular signaling pathways that are associated with migration of colorectal cancer cells, the cultures were exposed to selective inhibitors. In three out of five co

SUBMITTER: Lange F 

PROVIDER: S-EPMC10376471 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature