LSPR-Based Aptasensor for Rapid Urinary Detection of NT-proBNP.

ABSTRACT: N-terminal pro-brain natriuretic peptide (NT-proBNP) is a myocardial stress biomarker that can be found in serum or plasma, saliva, and urine in the context of cardiovascular disease. In this study, we developed a rapid (~25 min) and straightforward localized surface plasmon resonance (LSPR)-based assay for detecting NT-proBNP in urine. The assay employs citrate-capped gold nanoparticles (AuNPs) and an aptamer specific for NT-proBNP, which initially interacts with NT-proBNP. The remaining unbound aptamer then interacts with the AuNPs, and the addition of NaCl induces the aggregation of the unprotected AuNPs, resulting in a decrease in absorbance at the LSPR band (A₅₂₁) and an increase in absorbance at 750 nm (A₇₅₀). The concentration of NT-proBNP showed a linear corre

SUBMITTER: Antonio M 

PROVIDER: S-EPMC10377000 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature