Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells.

ABSTRACT: Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our

SUBMITTER: Pedrosa VA 

PROVIDER: S-EPMC10377447 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature