Amyloid β_1-42 Oligomers Induce Galectin-1^S8 O-GlcNAcylation Leading to Microglia Migration.

ABSTRACT: Protein O-GlcNAcylation has been associated with neurodegenerative diseases such as Alzheimer's disease (AD). The O-GlcNAcylation of the Amyloid Precursor Protein (APP) regulates both the trafficking and the processing of the APP through the amyloidogenic pathway, resulting in the release and aggregation of the Aβ_1-42 peptide. Microglia clears Aβ aggregates and dead cells to maintain brain homeostasis. Here, using LC-MS/MS, we revealed that the Aβ_1-42 oligomers modify the microglia O-GlcNAcome. We identified 55 proteins, focusing our research on Galectin-1 protein since it is a very versatile protein from a functional point of view. Combining biochemical with genetic approaches, we demonstrated that Aβ_1-42 oligomers specifically target Galectin-1^S8

SUBMITTER: Arrazola Sastre A 

PROVIDER: S-EPMC10378097 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature