Role of a Novel Heparanase Inhibitor on the Balance between Apoptosis and Autophagy in U87 Human Glioblastoma Cells.

ABSTRACT:

Background

Heparanase (HPSE) is an endo-β-glucuronidase that cleaves heparan sulfate side chains, leading to the disassembly of the extracellular matrix, facilitating cell invasion and metastasis dissemination. In this research, we investigated the role of a new HPSE inhibitor, RDS 3337, in the regulation of the autophagic process and the balance between apoptosis and autophagy in U87 glioblastoma cells.

Methods

After treatment with RDS 3337, cell lysates were analyzed for autophagy and apoptosis-related proteins by Western blot.

Results

We observed, firstly, that LC3II expression increased in U87 cells incubated with RDS 3337, together with a significant increase of p62/SQSTM1 levels, indicating that RDS 3337 could act through the inhibition of autophagic-lysosomal f

SUBMITTER: Manganelli V 

PROVIDER: S-EPMC10378526 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature