Optimization of Protocols for the Induction of Callus and Plant Regeneration in White Clover (Trifolium repens L.).

ABSTRACT: White clover is a widely grown temperate legume forage with high nutritional value. Research on the functional genomics of white clover requires a stable and efficient transformation system. In this study, we successfully induced calluses from the cotyledons and leaves of 10 different white clover varieties. The results showed that the callus formation rate in the cotyledons did not vary significantly among the varieties, but the highest callus formation rate was observed in 'Koala' leaves. Subsequently, different concentrations of antioxidants and hormones were tested on the browning rate and differentiation ability of the calluses, respectively. The results showed that the browning rate was the lowest on MS supplemented with 20 mg L^-1 AgNO₃ and 25 mg L^-1

SUBMITTER: Qi T 

PROVIDER: S-EPMC10378747 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature