Dataset Information

A Modified ¹H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

ABSTRACT: A previous ¹H-NMR method allowed the quantification of ephedrine alkaloids; however, there were some disadvantages. The cyclized derivatives resulted from the impurities of diethyl ether were identified and benzene was selected as the better extraction solvent. The locations of ephedrine alkaloids were confirmed with 2D NMR. Therefore, a specific ¹H-NMR method has been modified for the quantification of ephedrine alkaloids. Accordingly, twenty Ephedrae Herba samples could be classified into three classes: (I) E. sinica-like species; (II) E. intermedia-like species; (III) others (lower alkaloid contents). The results indicated that ephedrine and pseudoephedrine are the major alkaloids in Ephedra plants, but the concentrations vary greatly determined by the plant species and the collection locations.

SUBMITTER: Li YC

PROVIDER: S-EPMC10378770 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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A Modified 1H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

Li Yue-Chiun YC Wu Chia-Hung CH Le Thi Ha TH Yuan Qingjun Q Huang Luqi L Chen Guo-Fen GF Yang Mei-Lin ML Lam Sio-Hong SH Hung Hsin-Yi HY Sun Handong H Wu Yi-Hung YH Kuo Ping-Chung PC Wu Tian-Shung TS

International journal of molecular sciences 20230710 14

A previous 1H-NMR method allowed the quantification of ephedrine alkaloids; however, there were some disadvantages. The cyclized derivatives resulted from the impurities of diethyl ether were identified and benzene was selected as the better extraction solvent. The locations of ephedrine alkaloids were confirmed with 2D NMR. Therefore, a specific 1H-NMR method has been modified for the quantification of ephedrine alkaloids. Accordingly, twenty Ephedrae Herba samples could b ...[more]

PMID: 37511036

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Project description:The enhanced therapeutic effects and mechanisms of certain herbal combination in various herbal prescriptions are mostly unclear. A combination of two herbs, namely Ephedrae herba (EH) and Coicis semen (CS), has been commonly prescribed for obesity. In our previous work, the combination of EH and CS was studied using network pharmacological approach to predict its pharmacological targets and in vitro experiments to evaluate its efficacy on obesity. Although we demonstrated enhanced anti-adiposity effects of the combination on matured adipocytes, the molecular mechanisms and contributing compounds underlying the effects of EH-CS combination on adiposity or adipogenesis were not fully elucidated. The current study adopted integrated bioinformatics analysis to precisely validate potential targets of EH-CS by screening differentially expressed genes (DEGs) of morbid obesity patients from NCBI gene expression omnibus (GEO). Based on the functional cluster analysis of down-regulated DEGs, the anti-adipogenesis mechanism of EH-CS combination was speculated with KEGG enrichment analysis. Furthermore, we investigated the combinational effects of EH and coixol, or stigmasterol, the two compounds in CS which were expected to have main beneficial effects in metabolic diseases. Moreover, distinct effect of the combination on transcriptional activity of glucocorticoid receptor (GR) was investigated using electrophoretic mobility shift assay (EMSA). The EH-CS combination was predicted to modulate down-regulated genes which are involved in KEGG pathways crucial to metabolic disease in morbidly obese individuals. The combination of EH with CS compounds significantly increased the phosphorylation of acetyl-coA carboxylase (ACC), AMP-activated protein kinase (AMPK), and protein kinase B (AKT) in 3T3-L1 cells and decreased intracellular lipid accumulation. The two CS compounds significantly increased the anti-adipogenesis/lipogenesis effects of EH by inhibiting the gene expression levels. Finally, the combination of EH and coixol inhibited dexamethasone-induced GR translocation to the nucleus and transcriptional binding activity in adipocytes. The combination of EH and CS could be considered a therapeutic strategy for treating metabolic diseases, including obesity.

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A Modified ¹H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

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A Modified <sup>1</sup>H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

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Dataset Information

A Modified 1H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

Publications

A Modified &lt;sup&gt;1&lt;/sup&gt;H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

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OmicsDI is part of the ELIXIR infrastructure

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A Modified ¹H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.

A Modified <sup>1</sup>H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.