Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium.

ABSTRACT: Crypt-villus architecture in the small intestine is crucial for the structural integrity of the intestinal epithelium and maintenance of gut homeostasis. We utilized three-dimensional (3D) printing and inverse molding techniques to form three-dimensional (3D) spongy scaffold systems that resemble the intestinal crypt-villus microarchitecture. The scaffolds consist of silk fibroin protein with curved lumens with rows of protruding villi with invaginating crypts to generate the architecture. Intestinal cell (Caco-2, HT29-MTX) attachment and growth, as well as long-term culture support were demonstrated with cell polarization and tissue barrier properties compared to two-dimensional (2D) Transwell culture controls. Further, physiologically relevant oxygen gradients were generated in the 3D sy

SUBMITTER: Rudolph SE 

PROVIDER: S-EPMC10379436 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature