MiR-29a-3p Regulates Autophagy by Targeting Akt3-Mediated mTOR in SiO₂-Induced Lung Fibrosis.

ABSTRACT: Silicosis is a refractory pneumoconiosis of unknown etiology that is characterized by diffuse lung fibrosis, and microRNA (miRNA) dysregulation is connected to silicosis. Emerging evidence suggests that miRNAs modulate pulmonary fibrosis through autophagy; however, its underlying molecular mechanism remains unclear. In agreement with miRNA microarray analysis, the qRT-PCR results showed that miR-29a-3p was significantly decreased in the pulmonary fibrosis model both in vitro and in vivo. Increased autophagosome was observed via transmission electron microscopy in lung epithelial cell models and lung tissue of silicosis mice. The expression of autophagy-related proteins LC3α/β and Beclin1 were upregulated. The results from using 3-methyladenine, an autophagy inhibitor, or rapamycin, an auto

SUBMITTER: Li P 

PROVIDER: S-EPMC10380316 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature