Rapid Identification of Aphid Species by Headspace GC-MS and Discriminant Analysis.

ABSTRACT: Aphids are a ubiquitous group of pests in agriculture that cause serious losses. For sustainable aphid identification, it is necessary to develop a precise and fast aphid identification tool. A new simple chemotaxonomy approach to rapidly identify aphids was implemented. The method was calibrated in comparison to the established phylogenetic analysis. For chemotaxonomic analysis, aphids were crushed, their headspace compounds were collected through closed-loop stripping (CLS) and analysed using gas chromatography-mass spectrometry (GC-MS). GC-MS data were then subjected to a discriminant analysis using CAP12.exe software, which identified key biomarkers that distinguish aphid species. A dichotomous key taking into account the presence and absence of a set of species-specific biomarkers was

SUBMITTER: Alotaibi NJ 

PROVIDER: S-EPMC10380428 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature