Dataset Information

Reduced Cell Adhesion on LightPLAS-Coated Implant Surfaces in a Three-Dimensional Bioreactor System.

ABSTRACT: Most implants used in trauma surgery are made of steel and remain inside the body only temporarily. The strong tissue interaction of such implants sometimes creates problems with their explantation. Modified implant surfaces, which decrease tissue attachment, might allow an easier removal and therefore a better outcome. Such a modification must retain the implant function, and needs to be biocompatible and cost-effective. Here, we used a novel VUV-light (Vacuum-Ultraviolett)-based coating technology (LightPLAS) to generate coated stainless-steel plates. The tested LightPLAS coating only had an average thickness of around 335 nm, making it unlikely to interfere with implant function. The coated plates showed good biocompatibility according to ISO 10993-5 and ISO 10993-12, and reduced cell adhesion after four different time points in a 2D cell culture system with osteoblast-like MG-63 cells. Furthermore, we could show decreased cell adhesion in our 3D cell culture system, which mimics the fluid flow above the implant materials as commonly present in the in vivo environment. This new method of surface coating could offer extended options to design implant surfaces for trauma surgery to reduce cell adhesion and implant ingrowth. This may allow for a faster removal time, resulting in shorter overall operation times, thereby reducing costs and complication rates and increasing patient wellbeing.

SUBMITTER: Boker KO

PROVIDER: S-EPMC10380481 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Reduced Cell Adhesion on LightPLAS-Coated Implant Surfaces in a Three-Dimensional Bioreactor System.

Böker Kai Oliver KO Gätjen Linda L Dölle Christopher C Vasic Katarina K Taheri Shahed S Lehmann Wolfgang W Schilling Arndt Friedrich AF

International journal of molecular sciences 20230718 14

Most implants used in trauma surgery are made of steel and remain inside the body only temporarily. The strong tissue interaction of such implants sometimes creates problems with their explantation. Modified implant surfaces, which decrease tissue attachment, might allow an easier removal and therefore a better outcome. Such a modification must retain the implant function, and needs to be biocompatible and cost-effective. Here, we used a novel VUV-light (Vacuum-Ultraviolett)-based coating techno ...[more]

PMID: 37511369

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Project description:Bacterial biofilms reduce the performance and efficiency of biomedical and industrial devices. The initial step in forming bacterial biofilms is the weak and reversible attachment of the bacterial cells onto the surface. This is followed by bond maturation and secretion of polymeric substances, which initiate irreversible biofilm formation, resulting in stable biofilms. This implies that understanding the initial reversible stage of the adhesion process is crucial to prevent bacterial biofilm formation. In this study, we analyzed the adhesion processes of E. coli on self-assembled monolayers (SAMs) with different terminal groups using optical microscopy and quartz crystal microbalance with energy dissipation (QCM-D) monitoring. We found that a considerable number of bacterial cells adhere to hydrophobic (methyl-terminated) and hydrophilic protein-adsorbing (amine- and carboxy-terminated) SAMs forming dense bacterial adlayers while attaching weakly to hydrophilic protein-resisting SAMs [oligo(ethylene glycol) (OEG) and sulfobetaine (SB)], forming sparse but dissipative bacterial adlayers. Moreover, we observed positive shifts in the resonant frequency for the hydrophilic protein-resisting SAMs at high overtone numbers, suggesting how bacterial cells cling to the surface using their appendages as explained by the coupled-resonator model. By exploiting the differences in the acoustic wave penetration depths at each overtone, we estimated the distance of the bacterial cell body from different surfaces. The estimated distances provide a possible explanation for why bacterial cells tend to attach firmly to some surfaces and weakly to others. This result is correlated to the strength of the bacterium-substratum bonds at the interface. Elucidating how the bacterial cells adhere to different surface chemistries can be a suitable guide in identifying surfaces with a more significant probability of contamination by bacterial biofilms and designing bacteria-resistant surfaces and coatings with excellent bacterial antifouling characteristics.

Dataset Information

Reduced Cell Adhesion on LightPLAS-Coated Implant Surfaces in a Three-Dimensional Bioreactor System.

Publications

Reduced Cell Adhesion on LightPLAS-Coated Implant Surfaces in a Three-Dimensional Bioreactor System.

Similar Datasets

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