Cx43 Hemichannel and Panx1 Channel Modulation by Gap19 and ¹⁰Panx1 Peptides.

ABSTRACT: Cx43 hemichannels (HCs) and Panx1 channels are two genetically distant protein families. Despite the lack of sequence homology, Cx43 and Panx1 channels have been the subject of debate due to their overlapping expression and the fact that both channels present similarities in terms of their membrane topology and electrical properties. Using the mimetic peptides Gap19 and ¹⁰Panx1, this study aimed to investigate the cross-effects of these peptides on Cx43 HCs and Panx1 channels. The single-channel current activity from stably expressing HeLa-Cx43 and C6-Panx1 cells was recorded using patch-clamp experiments in whole-cell voltage-clamp mode, demonstrating 214 pS and 68 pS average unitary conductances for the respective channels. Gap19 was applied intracellularly while ¹⁰

SUBMITTER: Lissoni A 

PROVIDER: S-EPMC10380488 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature