Coregulators Reside within Drosophila Ecdysone-Inducible Loci before and after Ecdysone Treatment.

ABSTRACT: Ecdysone signaling in Drosophila remains a popular model for investigating the mechanisms of steroid action in eukaryotes. The ecdysone receptor EcR can effectively bind ecdysone-response elements with or without the presence of a hormone. For years, EcR enhancers were thought to respond to ecdysone via recruiting coactivator complexes, which replace corepressors and stimulate transcription. However, the exact mechanism of transcription activation by ecdysone remains unclear. Here, we present experimental data on 11 various coregulators at ecdysone-responsive loci of Drosophila S2 cells. We describe the regulatory elements where coregulators reside within these loci and assess changes in their binding levels following 20-hydroxyecdysone treatment. In the current study, we det

SUBMITTER: Krasnov AN 

PROVIDER: S-EPMC10380596 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature