Gold Particle Analyser: Detection and quantitative assessment of electron microscopy gold probes.

ABSTRACT: Gold particle probes are an essential electron microscopy tool to examine protein localisation, as well as protein trafficking. They can be introduced into living cells when conjugated to a protein that is endocytosed or to an antibody against a cell surface protein. Alternatively, gold particles can be introduced into fixed cells or tissue when conjugated to antibodies, immunoglobulin binding molecules or chemical probes applied to permeabilised samples or electron microscopy sections. Colloidal gold particles that have not been enlarged through chemical (gold or silver) enhancement are typically spherical and can be prepared in a range of specific sizes, allowing multiple proteins to be localised within a single sample. The typically homogeneous shape and size of the colloidal gold makes

SUBMITTER: Burgoyne T 

PROVIDER: S-EPMC10381077 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature