Host Range and Loop-Mediated Isothermal Amplification Detection of <i>Globisporangium sylvaticum</i> from Guizhou, China.

ABSTRACT: Globisporangium, especially G. sylvaticum, causes devastating root rot, blight, and other diseases in various species of cash crops. To investigate the distribution and host range of G. sylvaticum in Guizhou, a suitable habitat for this pathogen, we collected 156 root-diseased samples, isolated the pathogens, and found that G. sylvaticum is widespread and has eleven host plants, including four novel hosts. Furthermore, to effectively identify G. sylvaticum, we developed a simple and dependable method based on loop-mediated isothermal amplification (LAMP), which used a primer set designed from the internal transcribed spacer sequences with high specificity and sensitivity of 1 pg/μL. Additionally, to perform field identification, we used the "Plant-LAMP" m

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10381608 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature