Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.

ABSTRACT: The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A^Nuc). This revealed a central CENP-A^Nuc with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (C

SUBMITTER: Dendooven T 

PROVIDER: S-EPMC10381965 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature