Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity.

ABSTRACT:

Introduction

Understanding how photoreceptor genes are regulated is important for investigating retinal development and disease. While much is known about gene regulation in cones, the mechanism by which tandemly-replicated opsins, such as human long wavelength-sensitive and middle wavelength-sensitive opsins, are differentially regulated remains elusive. In this study, we aimed to further our understanding of transcriptional heterogeneity in cones that express tandemly-replicated opsins and the regulation of such differential expression using zebrafish, which express the tandemly-replicated opsins lws1 and lws2.

Methods

We performed bulk and single cell RNA-Seq of LWS1 and LWS2 cones, evaluated expression patterns of selected genes of interest using multiplex

SUBMITTER: Farre AA 

PROVIDER: S-EPMC10382231 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature