Preclinical evaluation of ⁶⁸Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma.

ABSTRACT:

Purpose

Hepatocellular carcinoma (HCC) is a highly vascularized solid carcinoma and tumor vessel-targeted molecular imaging might be effective for early diagnosis of HCC. Herein, we developed a novel trimeric affibody (Z_TRI) with highly specific binding to the platelet-derived growth factor receptor beta (PDGFRβ). The aim of this study is to evaluate the feasibility of ⁶⁸Ga-radiolabeled Z_TRI ([⁶⁸Ga]Ga-DOTA-Z_TRI) as PET tracer for diagnosis of HCC.

Methods

The bioinformatics analysis of clinical database and immunoblotting of clinical specimens were performed to validate the potential of PDGFRβ as HCC biomarker. The trimeric affibody Z_TRI was conjugated with DOTA-NHS-ester and radiolabeled with ⁶⁸Ga

SUBMITTER: Cai H 

PROVIDER: S-EPMC10382327 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature