Loss of Kruppel-like factor 9 deregulates both physiological gene expression and development.

ABSTRACT: Krüppel-like factor 9 (Klf9) is a ubiquitously expressed transcription factor that is a feedforward regulator of multiple stress-responsive and endocrine signaling pathways. We previously described how loss of Klf9 function affects the transcriptome of zebrafish larvae sampled at a single time point 5 days post-fertilization (dpf). However, klf9 expression oscillates diurnally, and the sampled time point corresponded to its expression nadir. To determine if the transcriptomic effects of the klf9^-/- mutation vary with time of day, we performed bulk RNA-seq on 5 dpf zebrafish embryos sampled at three timepoints encompassing the predawn peak and midmorning nadir of klf9 expression. We found that while the major effects of the klf9^-/- mutation that we reported previously

SUBMITTER: Drepanos L 

PROVIDER: S-EPMC10382561 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature