Two-Dimensional NMR Spectroscopy of the G Protein-Coupled Receptor A_2AAR in Lipid Nanodiscs.

ABSTRACT: Eight hundred and twenty-six human G protein-coupled receptors (GPCRs) mediate the actions of two-thirds of the human hormones and neurotransmitters and over one-third of clinically used drugs. Studying the structure and dynamics of human GPCRs in lipid bilayer environments resembling the native cell membrane milieu is of great interest as a basis for understanding structure-function relationships and thus benefits continued drug development. Here, we incorporate the human A_2A adenosine receptor (A_2AAR) into lipid nanodiscs, which represent a detergent-free environment for structural studies using nuclear magnetic resonance (NMR) in solution. The [¹⁵N,¹H]-TROSY correlation spectra confirmed that the complex of [u-¹⁵N, ~70% ²

SUBMITTER: Guo C 

PROVIDER: S-EPMC10383251 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature