Impact of the Food-Related Stress Conditions on the Expression of Enterotoxin Genes among Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is one of the most important foodborne pathogens. S. aureus has the capability to produce a variety of toxins, including staphylococcal enterotoxins (SEs). The aim of this study was to evaluate the survival rate of S. aureus cells and analyze enterotoxins gene expression after exposure to osmotic stress and acidic/alkaline stress. To determine survival rates, the traditional plate counting method and flow cytometry were used. The expression levels of the enterotoxin genes were performed by quantitative reverse transcription PCR (RT-qPCR). Expression changes differed depending on the stressors chosen. The obtained results in this study showed the effect of critical food-related stress conditions on SE gene expression in S. aureus. The study

SUBMITTER: Gajewska J 

PROVIDER: S-EPMC10383469 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature