Detection and Quantification of Ammonia as the Ammonium Cation in Human Saliva by ¹H NMR: A Promising Probe for Health Status Monitoring, with Special Reference to Cancer.

ABSTRACT: Ammonia (NH₃) has been shown to be a key biomarker for a wide variety of diseases, such as hepatic and chronic kidney diseases (CKD), and cancers. It also has relevance to the oral health research area, and, hence, its determination in appropriate biofluids and tissues is of much importance. However, since it contains exchangeable >N-H protons, its analysis via ¹H NMR spectroscopy, which is a widely employed technique in untargeted metabolomic studies, is rendered complicated. In this study, we focused on the ¹H NMR analysis of this biomarker in less invasively collected human saliva samples, and we successfully identified and quantified it as ammonium cation (NH₄⁺) in post-collection acidulated forms of this biofluid using both the st

SUBMITTER: Bhogadia M 

PROVIDER: S-EPMC10383521 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature