Shiga Toxin-Producing Escherichia coli (STEC) Associated with Calf Mortality in Uruguay.

ABSTRACT: In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. col

SUBMITTER: Fernandez M 

PROVIDER: S-EPMC10383947 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature