Nonenveloped Avian Reoviruses Released with Small Extracellular Vesicles Are Highly Infectious.

ABSTRACT: Vesicle-encapsulated nonenveloped viruses are a recently recognized alternate form of nonenveloped viruses that can avoid immune detection and potentially increase systemic transmission. Avian orthoreoviruses (ARVs) are the leading cause of various disease conditions among birds and poultry. However, whether ARVs use cellular vesicle trafficking routes for egress and cell-to-cell transmission is still poorly understood. We demonstrated that fusogenic ARV-infected quail cells generated small (~100 nm diameter) extracellular vesicles (EVs) that contained electron-dense material when observed by transmission electron microscope. Cryo-EM tomography indicated that these vesicles did not contain ARV virions or core particles, but the EV fractions of OptiPrep gradients did contain a small percent

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10384003 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature