Effects of Signal Peptide and Chaperone Co-Expression on Heterologous Protein Production in Escherichia coli.

ABSTRACT: Various host systems have been employed to increase the yield of recombinant proteins. However, some recombinant proteins were successfully produced at high yields but with no functional activities. To achieve both high protein yield and high activities, molecular biological strategies have been continuously developed. This work describes the effect of signal peptide (SP) and co-expression of molecular chaperones on the production of active recombinant protein in Escherichia coli. Extracellular enzymes from Bacillus subtilis, including β-1,4-xylanase, β-1,4-glucanase, and β-mannanase constructed with and without their signal peptides and intracellular enzymes from Pseudomonas stutzeri ST201, including benzoylformate decarboxylase (BFDC), benzaldehyde dehydrogenase (BAD

SUBMITTER: Jomrit J 

PROVIDER: S-EPMC10384211 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature