Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases.

ABSTRACT:

Background

Disturbed hepatic energy metabolism contributes to non-alcoholic fatty liver (NAFLD), but the development of changes over time and obesity- or diabetes-related mechanisms remained unclear.

Methods

Two-day old male C57BL/6j mice received streptozotocin (STZ) or placebo (PLC) and then high-fat (HFD) or regular chow diet (RCD) from week 4 (W4) to either W8 or W16, yielding control [CTRL = PLC + RCD], diabetes [DIAB = STZ + RCD], obesity [OBES = PLC + HFD] and diabetes-related non-alcoholic steatohepatitis [NASH = STZ + HFD] models. Mitochondrial respiration was measured by high-resolution respirometry and insulin-sensitive glucose metabolism by hyperinsulinemic-euglycemic clamps with stable isotope dilution.

Findings

NASH showed higher steatosis and NAFLD acti

SUBMITTER: Dewidar B 

PROVIDER: S-EPMC10384226 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature