Combination of RCA and DNAzyme for Dual-Signal Isothermal Amplification of Exosome RNA.

ABSTRACT: The RNA contained in exosomes plays a crucial role in information transfer between cells in various life activities. The accurate detection of low-abundance exosome RNA (exRNA) is of great significance for cell function studies and the early diagnosis of diseases. However, their intrinsic properties, such as their short length and high sequence homology, represent great challenges for exRNA detection. In this paper, we developed a dual-signal isothermal amplification method based on rolling circle amplification (RCA) coupled with DNAzyme (RCA-DNAzyme). The sensitive detection of low-abundance exRNA, the specific recognition of their targets and the amplification of the detection signal were studied and explored. By designing padlock probes to specifically bind to the target exRNA, while re

SUBMITTER: Xia Y 

PROVIDER: S-EPMC10384651 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature