Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing.

ABSTRACT: Here, we present a protocol for spatially annotated single-cell sequencing, a technique for spatially profiling intratumor heterogeneity with deep single-cell RNA sequencing and single-cell resolution. By combining live-cell imaging and photopatterned illumination, we describe steps to identify regions of interest in an in vitro tumor model, label the selected cells with photoactivatable dyes, and isolate and subject them to scRNAseq. This protocol can be applied to a range of cell lines and could be expanded to tissue sections. For complete details on the use and execution of this protocol, please refer to Smit et al. (2022).¹.

SUBMITTER: Smit MM 

PROVIDER: S-EPMC10384656 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature