Assessment of Different Niosome Formulations for Optogenetic Applications: Morphological and Electrophysiological Effects.

ABSTRACT: Gene therapy and optogenetics are becoming promising tools for treating several nervous system pathologies. Currently, most of these approaches use viral vectors to transport the genetic material inside the cells, but viruses present some potential risks, such as marked immunogenicity, insertional mutagenesis, and limited insert gene size. In this framework, non-viral nanoparticles, such as niosomes, are emerging as possible alternative tools to deliver genetic material, avoiding the aforementioned problems. To determine their suitability as vectors for optogenetic therapies in this work, we tested three different niosome formulations combined with three optogenetic plasmids in rat cortical neurons in vitro. All niosomes tested successfully expressed optogenetic channels, which were depend

SUBMITTER: Celdran JD 

PROVIDER: S-EPMC10384779 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature