Impact of Bacillus cereus on the Human Gut Microbiota in a 3D In Vitro Model.

ABSTRACT: In vitro models for culturing complex microbial communities are progressively being used to study the effects of different factors on the modeling of in vitro-cultured microorganisms. In previous work, we validated a 3D in vitro model of the human gut microbiota based on electrospun gelatin scaffolds covered with mucins. The aim of this study was to evaluate the effect of Bacillus cereus, a pathogen responsible for food poisoning diseases in humans, on the gut microbiota grown in the model. Real-time quantitative PCR and 16S ribosomal RNA-gene sequencing were performed to obtain information on microbiota composition after introducing B. cereus ATCC 14579 vegetative cells or culture supernatants. The adhesion of B. cereus to intestinal mucins was also tested. The presen

SUBMITTER: Calvigioni M 

PROVIDER: S-EPMC10385275 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature