A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology.

ABSTRACT: The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic Leptospira lipL32 gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the lipL32 gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test

SUBMITTER: Natarajan S 

PROVIDER: S-EPMC10385553 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature