A Novel, Precise and High-Throughput Technology for Viroid Detection in Cannabis (MFDetect^TM).

ABSTRACT: Hop latent viroid (HLVd) is a severe disease of cannabis, causing substantial economic losses in plant yield and crop value for growers worldwide. The best way to control the disease is early detection to limit the spread of the viroid in grow facilities. This study describes MFDetect^TM as a rapid, highly sensitive, and high-throughput tool for detecting HLVd in the early stages of plant development. Furthermore, in the largest research study conducted so far for HLVd detection in cannabis, we compared MFDetect^TM with quantitative RT-PCR in a time course experiment using different plant tissues, leaves, petioles, and roots at different plant developmental stages to demonstrate both technologies are comparable. Our study found leaf tissue is a suitable plant material f

SUBMITTER: Fernandez I Marti A 

PROVIDER: S-EPMC10385567 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature