Deacetylation of FOXP1 by HDAC7 potentiates self-renewal of mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) are widely used in a variety of tissue regeneration and clinical trials due to their multiple differentiation potency. However, it remains challenging to maintain their replicative capability during in vitro passaging while preventing their premature cellular senescence. Forkhead Box P1 (FOXP1), a FOX family transcription factor, has been revealed to regulate MSC cell fate commitment and self-renewal capacity in our previous study.

Methods

Mass spectra analysis was performed to identify acetylation sites in FOXP1 protein. Single and double knockout mice of FOXP1 and HDAC7 were generated and analyzed with bone marrow MSCs properties. Gene engineering in human embryonic stem cell (hESC)-derived MSCs was obtained to evaluate the impact o

SUBMITTER: Ling S 

PROVIDER: S-EPMC10385979 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature