Preparation of Alcohol Dehydrogenase-Zinc Phosphate Hybrid Nanoflowers through Biomimetic Mineralization and Its Application in the Inhibitor Screening.

ABSTRACT: A biomimetic mineralization method was used in the facile and rapid preparation of nanoflowers for immobilizing alcohol dehydrogenase (ADH). The method mainly uses ADH as an organic component and zinc phosphate as an inorganic component to prepare flower-like ADH/Zn₃(PO₄)₂ organic-inorganic hybrid nanoflowers (HNFs) with the high specific surface area through a self-assembly process. The synthesis conditions of the ADH HNFs were optimized and its morphology was characterized. Under the optimum enzymatic reaction conditions, the Michaelis-Menten constant (K_m) of ADH HNFs (β-NAD⁺ as substrate) was measured to be 3.54 mM, and the half-maximal inhibitory concentration (IC₅₀) of the positive control ranitidine (0.2-0.8 mM)

SUBMITTER: Luo ML 

PROVIDER: S-EPMC10386709 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature