Dapagliflozin-affected endothelial dysfunction and altered gut microbiota in mice with heart failure.

ABSTRACT:

Aim

To investigate the potential microbiome profile of a mouse model with heart failure (HF) during dapagliflozin treatment.

Method

An HF model was constructed in 8-week-old male mice, and cardiac tissues were analyzed using histological staining. Hemodynamic indexes were measured, and fecal samples were collected for 16S rDNA sequencing. Chao1, Shannon, and Simpson were used for α-diversity analysis. b-Diversity analysis was conducted using principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) based on the Bray-Curtis distance. Linear discriminant analysis coupled with effect size measurements (LEfSe) was used to identify signature gut microbiota, and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was

SUBMITTER: Bao N 

PROVIDER: S-EPMC10386824 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature