Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response.

ABSTRACT:

Background

The Echinococcus granulosus sensu lato species complex causes cystic echinococcosis, a zoonotic disease of medical importance. Parasite-derived small extracellular vesicles (sEVs) are involved in the interaction with hosts intervening in signal transduction related to parasite proliferation and disease pathogenesis. Although the characteristics of sEVs from E. granulosus protoscoleces and their interaction with host dendritic cells (DCs) have been described, the effect of sEVs recovered during parasite pharmacological treatment on the immune response remains unexplored.

Methods

Here, we isolated and characterized sEVs from control and drug-treated protoscoleces by ultracentrifugation, transmission electron microscopy, dynamic light scattering, and proteomic analys

SUBMITTER: Nicolao MC 

PROVIDER: S-EPMC10387209 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature