Human precision-cut cystic duct and gallbladder slices: a novel method for studying cholangiopathies.

ABSTRACT:

Background and aims

Precision-cut tissue slices (PCTS) are widely used as an ex vivo culture tissue culture technique to study pathogeneses of diseases and drug activities in organs in vitro. A novel application of the PCTS model may be in the field of translational research into cholangiopathies such as biliary atresia. Therefore, the aim of this study was to apply the precision-cut slice technique to human bile duct and gallbladder tissue.

Methods

Cystic duct and gallbladder tissue derived from patients undergoing a cholecystectomy were collected, preserved and used for preparation of precision-cut cystic duct slices (PCCDS) and precision-cut gallbladder slices (PCGS). The PCCDS and PCGS were prepared using a mechanical tissue slicer and subsequently incubate

SUBMITTER: Fridrichs J 

PROVIDER: S-EPMC10387522 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature