LINC00460 mediates HMGA2 expression through binding to miRNA-143-5p competitively in gastric carcinoma.

ABSTRACT:

Background

Compelling evidence has manifested a strong association between aberrant expression of long noncoding RNAs (lncRNAs) and gastric carcinoma (GC) development. Nonetheless, biological impacts of differentially expressed lncRNAs (DElncRNAs) on GC are not scrutinized.

Methods

Bioinformatics methods were employed for differential expression analysis and target gene prediction. MTT, colony formation, and Transwell methods were implemented for GC cell proliferation, migration, and invasion assessment. Western blot was implemented to test the protein level. The binding of genes was tested with dual-luciferase and RNA binding protein immunoprecipitation (RIP) approaches.

Results

Noticeably high level of LINC00460 was observed in GC tissues and cells. LINC00460 silenc

SUBMITTER: Zhu X 

PROVIDER: S-EPMC10387914 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature