SmFRET Detection of Cis and Trans DNA Interactions by the BfiI Restriction Endonuclease.

ABSTRACT: Protein-DNA interactions are fundamental to many biological processes. Proteins must find their target site on a DNA molecule to perform their function, and mechanisms for target search differ across proteins. Especially challenging phenomena to monitor and understand are transient binding events that occur across two DNA target sites, whether occurring in cis or trans. Type IIS restriction endonucleases rely on such interactions. They play a crucial role in safeguarding bacteria against foreign DNA, including viral genetic material. BfiI, a type IIS restriction endonuclease, acts upon a specific asymmetric sequence, 5-ACTGGG-3, and precisely cuts both upper and lower DNA strands at fixed locations downstream of this sequence. Here, we present two single-molecule Förster resonance energy-t

SUBMITTER: Ivanovaite SN 

PROVIDER: S-EPMC10388346 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature