TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis.

ABSTRACT: Research in neuroscience has evolved to use complex imaging and computational tools to extract comprehensive information from data sets. Calcium imaging is a widely used technique that requires sophisticated software to obtain reliable results, but many laboratories struggle to adopt computational methods when updating protocols to meet modern standards. Difficulties arise due to a lack of programming knowledge and paywalls for software. In addition, cells of interest display movements in all directions during calcium imaging. Many approaches have been developed to correct the motion in the lateral (x/y) direction. This paper describes a workflow using a new ImageJ plugin, TrackMate Analysis of Calcium Imaging (TACI), to examine motion on the z-axis in 3D calcium imaging. This software ide

SUBMITTER: Omelchenko AA 

PROVIDER: S-EPMC10388512 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature