Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA.

ABSTRACT:

Background

Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase.

Objective

To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer. We then sought to determine the stability at room temperature of the premixed LAMP reagents containing sucrose.

Method

Premixed LAMP reagents with sucrose and without sucrose were prepared. The prepared mixtures were stored at room temperature for up to 60 days, and were subjected to LAMP reactions at various intervals using rat kidney samples to detect leptospiral DNA.

Results

The pre

SUBMITTER: Lee PY 

PROVIDER: S-EPMC10388737 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature