A novel transillumination meibography device for in vivo imaging of mouse meibomian glands.

ABSTRACT:

Purpose

While mouse models of dry eye disease (DED) have been developed, studies evaluating the role of the meibomian glands limited by the inability to temporally document changes. In this report we describe the development of a novel mouse transillumination meibography device and assess the ability of this device to detect age-related changes in the meibomian glands of young and old mice.

Methods

The mouse meibography device was comprised of a 3 mm wide right angle prism attached to broad spectrum light source by an optical fiber. Eyelids were then pulled over the prism using double tooth forceps and imaged using a stereomicroscope and low light level camera. Meibomian glands from four young and four old male, BALB/c mice were then imaged and analyzed using ImageJ.

Resu

SUBMITTER: Hwang HS 

PROVIDER: S-EPMC10388835 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature