Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.).

ABSTRACT: Rice (Oryza sativa L.) is a staple food in many countries around the world, particularly in China. The production of rice is seriously affected by the bacterial leaf streak and rice blast, which can reduce rice yield or even cause it to fail to be harvested. In this study, susceptible material 58B was edited by CRISPR/Cas9, targeting a target of the Pi21 gene and a target of the effector-binding element (EBE) of the OsSULTR3;6 gene, and the mutants 58b were obtained by Agrobacterium-mediated method. The editing efficiency of the two targets in the T₀ generation was higher than 90.09%, the homozygous mutants were successfully selected in the T₀ generation, and the homozygous mutation rate of each target was higher than 26.67%. The expression

SUBMITTER: Yang J 

PROVIDER: S-EPMC10389665 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature