Comparison of denaturing agent effects in enzymatic N-glycan release for human plasma N-glycan analysis.

ABSTRACT: Glycosylation is an essential posttranslational modification observed in the living proteome. Glycosylation profiles in glycoproteins can change in commonly observed diseases such as cancer. Identifying these changes is crucial in discovering new biomarkers for the early diagnosis of cancer. One of the main steps of N-glycan analysis is to release N-glycans from glycoproteins by specific enzymes. The study compares common denaturing agent combinations used in N-glycan release methods. In the study, human plasma was used to test the release methods of N-glycans containing different detergent combinations. The released N-glycans were labeled with the procainamide tag, purified using cellulose-containing solid-phase extraction cartridges, and analyzed by hig

SUBMITTER: Kayili HM 

PROVIDER: S-EPMC10390200 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature