Effect of four fluoroquinolones on the viability of bladder cancer cells in 2D and 3D cultures.

ABSTRACT:

Introduction

The anticancer properties of fluoroquinolones and the high concentrations they achieve in urine may help in bladder cancer therapy. This study aimed to analyze the properties of 4 fluoroquinolones as potential candidates for supportive treatment of bladder cancer.

Methods

Comparative analyses were performed on the cytotoxic effects of norfloxacin, enrofloxacin, moxifloxacin, and ofloxacin on normal and cancer urothelial cell lines. In 2D culture, the cytotoxic properties of fluoroquinolones were evaluated using MTT assay, real-time cell growth analysis, fluorescence and light microscopy, flow cytometry, and molecular analysis. In 3D culture, the properties of fluoroquinolones were tested using luminescence assays and confocal microscopy.

Results and discussio

SUBMITTER: Kloskowski T 

PROVIDER: S-EPMC10390741 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature