High throughput Luminex beads based multiplex assay for identification of six major bacterial pathogens of mastitis in dairy animals.

ABSTRACT:

Introduction

Bovine mastitis is caused by over 150 different microorganisms. Specific identification and quantification of multiple bacteria in a single milk sample becomes essential for rapid intervention.

Methods

In the present study a Luminex beads based multiplex assay emphasizing on the precise identification of six major bacterial pathogens of mastitis was developed. Assay was developed in two triplex sets, triplex 1 comprised of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis while triplex 2 consisted of Staphylococcus aureus, E. coli and Klebsiella pneumoniae.

Results

The analytical sensitivity was 10 6 copies per reaction mixture for all the six bacteria. A 100% analytical specificity was

SUBMITTER: Shrinet G 

PROVIDER: S-EPMC10390833 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature