A structured evaluation of cryopreservation in generating single-cell transcriptomes from cerebrospinal fluid.

ABSTRACT: Single-cell transcriptomics allows characterization of cerebrospinal fluid (CSF) cells at an unprecedented level. Here, we report a robust cryopreservation protocol adapted for the characterization of fragile CSF cells by single-cell RNA sequencing (RNA-seq) in moderate- to large-scale studies. Fresh CSF was collected from twenty-one participants at two independent sites. Each CSF sample was split into two fractions: one was processed fresh, while the second was cryopreserved for months and profiled after thawing. B and T cell receptor sequencing was also performed. Our comparison of fresh and cryopreserved data from the same individuals demonstrates highly efficient recovery of all known CSF cell types. We find no significant difference in cell type proportions and cellular transcriptomes

SUBMITTER: Touil H 

PROVIDER: S-EPMC10391561 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature