Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells.

ABSTRACT: Emerging evidence of species divergent features of astrocytes coupled with the relative inaccessibility of human brain tissue underscore the utility of human pluripotent stem cell (hPSC) technologies for the generation and study of human astrocytes. However, existing approaches for hPSC-astrocyte generation are typically lengthy or require intermediate purification steps. Here, we establish a rapid and highly scalable method for generating functional human induced astrocytes (hiAs). These hiAs express canonical astrocyte markers, respond to pro-inflammatory stimuli, exhibit ATP-induced calcium transients and support neuronal network development. Moreover, single-cell transcriptomic analyses reveal the generation of highly reproducible cell populations across individual donors, mostly resem

SUBMITTER: Berryer MH 

PROVIDER: S-EPMC10391684 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature