Targeted screening and identification of chlorhexidine as a pro-myogenic circadian clock activator.

ABSTRACT:

Background

The circadian clock is an evolutionarily conserved mechanism that exerts pervasive temporal control in stem cell behavior. This time-keeping machinery is required for orchestrating myogenic progenitor properties in regenerative myogenesis that ameliorates muscular dystrophy. Here we report a screening platform to discover circadian clock modulators that promote myogenesis and identify chlorhexidine (CHX) as a clock-activating molecule with pro-myogenic activities.

Methods

A high-throughput molecular docking pipeline was applied to identify compounds with a structural fit for a hydrophobic pocket within the key circadian transcription factor protein, Circadian Locomotor Output Cycles Kaput (CLOCK). These identified molecules were further screened for clock-modulato

SUBMITTER: Kiperman T 

PROVIDER: S-EPMC10391781 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature