Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat.

ABSTRACT:

Background

Induction of mutation through chemical mutagenesis is a novel approach for preparing diverse germplasm. Introduction of functional alleles in the starch biosynthetic genes help in the improvement of the quality and yield of cereals.

Results

In the present study, a set of 350 stable mutant lines were used to evaluate dynamic variation of the total starch contents. A megazyme kits were used for measuring the total starch content, resistant starch, amylose, and amylopectin content. Analysis of variance showed significant variation (p < 0.05) in starch content within the population. Furthermore, two high starch mutants (JE0173 and JE0218) and two low starch mutants (JE0089 and JE0418) were selected for studying different traits. A multiple comparison test showed that

SUBMITTER: Irshad A 

PROVIDER: S-EPMC10391901 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature